This book focuses on bioanalytical applications in the multidisciplinary and growing field of nanopore research. Based on the proceedings of the 1st International Conference on Nanopores for Bioanalytical Applications (Lanzarote, 2012), it is suitable for readers in both academia and industry. It describes the latest experimental and fabrication protocols necessary to carry out nanopore-based experiments and covers a wide variety of topics. These include: ion transport; polymers in solution; translocation processes; electrophoresis and mobility; hydrodynamics; cell design; noise as an analytical tool; pore characterization; fabrication methods; DNA and RNA translocation; protein binding; DNA unzipping; sequence analysis, and molecular gating.