Environmental Responses in Plants: Methods and Protocols

PART I Tropisms and OTHER Developmental responses

1 Analysis of Plant Root Gravitropism

Richard Barker, Sarah Johns, Ralph Trane, and Simon Gilroy

2 Hydrotropism: Analysis of the Root Response to a Moisture Gradient

Irene García-Maquilón, Jorge Lozano-Juste, Abdulwahed F. Alrefaei, and Pedro L. Rodriguez

3 Evaluating Root Mechanosensing Response in Rice

Tiago F. Lourenço, André M. Cordeiro, João Frazão, Nelson J.M. Saibo, and M. Margarida Oliveira

4 Physiological Analysis of Phototropic Responses to Blue and Red Light in Arabidopsis

Mathias Zeidler

5 Shoot Branching Phenotyping in Arabidopsis and Tomato

Ana Confraria, Aitor Muñoz-Gasca, Liliana Ferreira, Elena Baena-González, and Pilar Cubas

6 Ribo-Seq Method to Study Genome-Wide Translational Regulation in Plants

Hao Chen, Jose M. Alonso, and Anna N. Stepanova

PART II Photoperiodism and Circadian Rythms

7 Assessing Flowering Time Under Different Photoperiods

Jesús Praena, Elizabeth van Veen, Rossana Henriques, and Reyes Benlloch

8 Analysis of Phytochrome-Dependent Seed Germination in Arabidopsis

Mathias Zeidler

9 Rhythmic Leaf and Cotyledon Movement Analysis

Ping Lou, Kathleen Greenham, and C. Robertson McClung

10 Assessing Plant Pigment Regulation in Circadian Experiments

Raquel Esteban, Víctor Resco de Dios, and José Ignacio García-Plazaola

11 Chemical perturbation of Chloroplast Ca2+ Dynamics in Arabidopsis thaliana Suspension Cell Cultures and Seedlings

Panfeng Yao, Steffen Vanneste, Lorella Navazio, Frank Van Breusegem, and Simon Stael

PART III Abiotic Stress Responses

12 Screening for Abiotic Stress Response in Rice

Telma Fernandes, Fredilson Melo, Maria Beatriz Vieira, Tiago F. Lourenço, Chiara Pucciariello, Nelson J. M. Saibo, Isabel A. Abreu, and M. Margarida Oliveira

13 Etiolated Hypocotyls: A New System to Study the Impact of Abiotic Stress on Cell Expansion

Guiomar Martín and Paula Duque

14 Assessing Postgermination Development in Arabidopsis thaliana under Abiotic Stress

Rui Albuquerque-Martins, Alba Rodríguez Díez, Dóra Szakonyi, and Paula Duque

15 Assessing Abscisic Acid-Mediated Changes in Stomatal Aperture Through High-Quality Leaf Impressions

Alba Rodríguez Díez, Paula Duque, and Rossana Henriques

16 Evaluation of the Anti-Transpirant Activity of ABA-Receptor Agonists in Monocot and Eudicot Plants

Daniel Franco-Aragón, Irene García-Maquilón, Alfredo Manicardi, Pedro L. Rodríguez, and Jorge Lozano-Juste

17 Quantifying Phytohormones In Vivo with FRET Biosensors and the FRETENATOR Analysis Toolset

James H. Rowe, Annalisa Rizza, and Alexander M. Jones

18 Optical Imaging Resources for Crop Phenotyping and Stress Detection

Phatchareeya Waiphara, Cyril Bourgenot, Lindsey J Compton, and Ankush Prashar